品牌：W.沙利文
基本信息
·出版社：科学出版社
·ISBN：7030124405
·条码：9787030124401
·版次：1
·装帧：平装
内容简介
果蝇是遗传学、发育学研究的重要模式生物之一，对于发育生物学、基因组学领域中的探索具有极为重要的意义。本书是冷臬港实验室出版社Drosophila Protocols的影印版，共提供了近40个未来10年内最可能用到的以果蝇为实验对象的分子生物学、生物化学和细胞生物学研究的实验方案。每一个方案都经过专家的精心挑选和雕琢，实验设计严谨、准确、简洁、规范，可操作性强，值得称道。方案涉及染色体、细胞、基因组和发育等方面，既包含了初学者需要了解的基础知识，也涵盖了资深研究者所需的细节。本书的版式设计侧重于方便读者使用，正文中穿插了丰富的图表作为实验设计的辅助说明，附录中还列出了果蝇研究所需的重要资料如基本溶液、缓冲液的配方和配制方法等。
本书适合于从事发育生物学、细胞生物学、基因组学、遗传学、分子生物学、药物设计和开发以及功能基因学组研究的相关教学科研人员以及相关学科的本科生、研究生参考使用。
目录
Preface
Abbreviations
CHROMOSOMES
1 Preparation and Analysis of Drosophila Mitotic Chromosomes
2 In Situ Hybridization to Somatic Chromosomes
3 BrdU Labeling of Chromosomes
4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy
5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster
6 Preparation and Analysis of Polytene Chromosomes
7 In Situ Hybridizaiton to Polytene Chromosomes
8 Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
CELL BIOLOGY
9 Fluorescent Analysis of Drosophila Embryos
10 Imaginal Discs
11 The Neuromuscular Junction
12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa
13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult
14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy
15 Electrophysiological Approaches to the Neuromusculature
16 Functional Assays of the Peripheral and Central Nervous Systems
17 GFP and Other Reporters
18 Quantitative Microinjection of Drosophila Embryos
19 Targeted Disruption of Gene Function in Drosophila by RNA Interference
20 Drosophila Cell Culture and TRransformation
21 Generating Antibodies against Drosophila Proteins
22 Photoactivated Gene Expression for Cell-fate Mapping and Cell Manipulation
MOLECULAR BIOLOGY
23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue
24 GAL4-mediated Ectopic Gene Expression in Drosophila
25 Functional Cell Ablation
GENOMICS
26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones
27 Drosophila Microarrays:From Arrayer Construction to Hybridization
28 Using Drosophila Genome Databases
29 Preparation and Analysis of High-molecular-weight DNA in Drosophila
BIOCHEMISTRY
30 Culturing Large Populations of Drosophila for Protein Biochemistry
31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis
32 Preparation of Membrane Proteins from Drosophila Embryos
33 Preparing Cytoplasmic Extracts from Drosophila Embryos
34 Immunoblotting of Proteins from Single Drosophila Embryos
THE ORGANISM
35 Laboratory Culture of Drosophila
36 Preparation of Larval and Adult Cuticles for Light Microscopy
37 Exposing Drosophila to Neuroactive Drugs
APPENDICES
1 1989 Table of Contents from Drosophila:A Laboratory Manual
2 Anatomical Drawings of Drosophila
3 Solutions and Reagents
4 Cautions
5 Suppliers
6 Trademarks
Index
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