Product Description
High-throughput processing and purification
Insect RoboPop™ Ni-NTA His•Bind® Purification Kit is designed for filtration-based 96-well format purification of His•Tag® fusion proteins directly from transfected cultures of insect cells without cell harvest. This kit includes Insect PopCulture® Reagent for protein extraction from total transfected cultures, Benzonase® Nuclease for viscosity reduction, Ni-NTA His•Bind resin and buffers, 2-ml 96-well Filter Plate and Collection Plate with Sealer. The Insect PopCulture method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and protein yields (1). Use the kit with insect cells grown in suspension or adherent cells grown on tissue culture plates.
The kit is configured for robotic processing of transfected 10-ml suspension cultures and purifies up to 400 µg His•Tag fusion protein per culture based on binding capacity of the resin. Protein yields ranging from 60 µg to 140 µg per 10 ml cultures were obtained with β-gal, Fluc, MAP kinase and cdc2 kinase expressed in the pIEx™ transient protein expression system. Insect PopCulture Reagent is added directly to the suspension cell culture, followed by the addition of Benzonase Nuclease. The lysate is transferred to an appropriate size tube or deep-well plate and Ni-NTA His•Bind resin is added. After mixing, the lysate-resin slurry is transferred to the filter plate, and washing and elution steps are carried out on a vacuum manifold. The Filter Plate is compatible with standard vacuum manifolds for either manual or automated processing, and the entire purification has been validated for robotic sample processing with the MultiPROBE® II liquid handling workstation from PerkinElmer Life Sciences.
Also use the Insect RoboPop Ni-NTA His•Bind Purification Kit with centrifugation when a vacuum manifold is not available.
參考答案:产品说明
高通量纯化工艺昆虫
robopop™镍旅游●约束自己®净化过滤袋设计的96孔纯化格式 他—®标签融合蛋白直接从基因细胞培养昆虫细胞无收获. 本套包括昆虫Popculture®试剂提取总蛋白转文化benzonase®核酸为降粘, 镍旅游树脂和约束自己●缓冲期,296毫升阱过滤板、收藏板封闭. 昆虫Popculture法复苏中蛋白释放到细胞内蛋白质中, 加工效率提高产量和蛋白质(1). 使用这套教材与昆虫细胞悬浮或贴壁细胞生长在组织培养板. 该套配置的机器人加工转10毫升悬浮培养和纯化高达400µ其政●标签融合 每蛋白结合能力的培养基于树脂. 蛋白质产量从60µ至140克,每克10毫升µ文化得到β-乳糖、氟康唑、 MAP激酶和cdc2激酶的表达piex™系统暂态表达. 昆虫Popculture试剂加上直接受细胞培养,然后添加benzonase核酸. 转到一个适当的规模是裂解管或深好板块和镍旅游●约束其加入树脂. 搅拌后,裂解树脂浆转到过滤板 洗涤和洗脱步骤和进行多方面的真空. 符合标准的真空过滤机板或是手动或自动流加工 整个净化和机器人已审定样加工与多探头®Ⅱ由液体处理工作站PerkinElmer 生命科学. 还利用昆虫robopop镍旅游●约束其与纯化试剂盒在真空离心流不详.
有些术语太难了，需要英语达人来翻译，不过我把它翻译成这样也基本可以了！呵呵
