Lipid Rafts and Caveolae: From Membrane Biophysics to Cell Biology膜脂筏与细胞膜穴样内陷：从膜生物物理学到细胞生物学

作者： Christopher J. Fielding著

出 版 社：

出版时间： 2006-3-1字数：版次：页数： 278印刷时间： 2006/03/01开本： 16开印次：纸张： 胶版纸I S B N ： 9783527312610包装： 精装内容简介

This keenly awaited first overview of the field represents a complete guide to the structure and function of the most important mammalian cell membrane organelles. Filling a huge gap in the primary literature, this book is the first to cover the subject in detail.

Following an introduction by Kai Simons, the discoverer of lipid rafts and the most prominent scientist in the field, chapters include:

Historical background;

Distinct structures and functions;

Structural basis;

Signaling;

Viral entry and virion budding;

Cholesterol transport;

Caveolins;

Lipid shells;

Cell polarity and intracellular trafficking;

Cancer cells;

Of prime importance to molecular and cell biologists, biochemists, membrane scientists, cancer researchers, and virologists.

作者简介：

Christopher Fielding is Neider Professor of Cardiovascular Physiology at the University of California at San Francisco (UCSF). He graduated from University College in London (UK) where he also received his PhD. After appointments at Oxford University and at the University of Chicago, he joined the faculty at UCSF in 1971, being appointed full professor in 1985.

Professor Fielding's main research interest is in the trafficking of cholesterol, its regulation and its role in signal transduction.

目录

Table of Contents

1 Lipid Rafts, Caveolae, and Membrane Traffic

Doris Meder and Kai Simons

1.1Introduction

1.2Basic Organization Principles of a Cell Membrane

1.3Evidence for Phase Separation in Model Membrane Systems: Liquid-Ordered and Liquid-Disordered Phases

1.4Evidence for Phase Separation in Cell Membranes: The "Raft Concept"

1.5Raft Domains are Clustered to Exert their Function

1.6The Apical Membrane of Epithelial Cells: A Percolating Raft Membrane at 25 ~C

1.7Caveolae: Scaffolded Membrane Domains Rich in Raft Lipids

1.8Caveolae and Lipid Rafts in Membrane Traffic

Abbreviations

References

2 The Forces that Shape Caveolae

Pierre Sens and Matthew S. Turner

2.1Introduction

2.2Physical Modeling of Lipid Membranes

2.3Caveolae as Invaginated Lipid Rafts

2.4Membrane Inclusions

2.5Caveolae as a Thermodynamic Phase Separation of Membrane Proteins

2.6Caveolae and Membrane Tension: Meehano-Sensitivity and Mechano-Regulation

2.7Conclusions

Abbreviations

References

3The Biophysical Characterization of Lipid RaFts

Pranav Sharma, Rajat Varma, and Satyajit Mayor

3.1Introduction: The Fluid Mosaic Model and Membrane Domains

3.2The Origin of the Raft Hypothesis

3.3The Role of Lipid-Anchored Proteins in the Development of the Membrane Raft Hypothesis

3.4The Case For and Against DRMs as Evidence for "Rafts" in Cell Membranes

3.5Why Are Biophysical Studies Useful for Understanding Lipid Rafts?

3.6Diffusion-Based Measurements

3.6.1Single-Molecule Studies

3.6.2Fluorescence Recovery After Photobleachin2

3.6.3Fluorescence Correlation Spectroscopy

3.7Proximity Measurements

3.7.1Proximity Measurement Using Homo-FRET

3.7.2Proximity Measurement Using Hetero-FRET

3.8Conclusions

Abbreviations

References

4 The Role of Caveolae and Noncaveolar Rafts in Endocytosis

Bo van Deurs, Frederilc Vilhardt, Maria Torgersen, Kirstine Roepstorff, Anette M. Hommelgaard, and Kirsten Sandvig

4.1Introduction

4.2Caveolae are Largely Immobile, Nonendocytic Membrane Domains

4.3Caveolae May Show Local, Short-Range Motility: A Role in Transendothelial Transport?

4.4An Internalization Wave of Caveolae can be Stimulated by Virus

4.5Role of Caveolae in Endocytosis of Cholera Toxin

4.6A Small Fraction of Caveolae may become Constitutively Internalized

4.7Caveosomes: Intracellular Caveolin-Associated Structures

4.8The Role of Dynamin in Caveolar Function

4.9Caveolin Immobilizes Rafts/Caveolar Invaginations

4.10A 2005 Consensus Model for Caveolar Endocytosis

Acknowledgments

Abbreviations

References

5Role of Cholesterol in Signal Transduction from Caveolae

Christopher J. FieLding and Phoebe E. Fidding

5.1Introduction

5.2Lipids of Caveolae

……

6 Phosphorylation of Caveolin and Signaling from Caveolae

7 Role of Lipid Microdomains in the Formation of Supramolecular Protein Cp,[;exes amd Trams,e,brame Signaling

8 Caveolin and its Role in Intracellular Chaperone Complexes

9 The Roles of Caveolae and Caveolin in Cell Shape,Locomotion,and Stress Fiber Formation

10 Lipid Rafts in Trafficking and Processing of Prion Protein and Amyloid Precursor Protein

11 Caveolae and the Endothelial Nitric Oxide Synthase

12 The Role of Caveolin-1 in Tumor Cell Survival and Cancer Progression

Index