后基因组技术进展:第6届国际后基因组生命科学技术学术论坛论文集(英文版)
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品牌: 罗国安
基本信息·出版社：东南大学出版社
·页码：459 页
·出版日期：2009年10月
·ISBN：9787564118426
·条形码：9787564118426
·包装版本：第1版
·装帧：平装
·开本：16
·正文语种：英语
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内容简介《后基因组技术进展:第6届国际后基因组生命科学技术学术论坛论文集(英文版)》内容简介：Human body consists of many organs where many cells work together to perform their functions. Although massive molecular data are averages over ensembles of cells, the question is whether all the cells in an organ work in the same way. In a cancer tissue, it has been pointed out that a small amount of cancer stem cells play important roles to reconstruct a cancer tissue again even after destroying the cancer tissue by anti-cancer drugs.
编辑推荐《后基因组技术进展:第6届国际后基因组生命科学技术学术论坛论文集(英文版)》由东南大学出版社出版。
目录
KEYNOTE LECTURES
NOVEL SEPARATION AND IDENTIFICATION TECHNIQUES FOR PROTEOME STUDY
APPLICATION OF RNA CHECK: GENE EXPRESSION PROFILES OF DISSECTED SPECIMEN FOR
PREDICTION OF METASTASES OF COLON CANCER
NUCLEIC ACID MASS SPECTROMETRY: A TECHNIQUE WITH UNUSUAL SENSITIVITY AND PRECISION
DEVELOPMENT OF DNA ANALYSIS TECHNOLOGIES ——WHAT COMES NEXT——
THE PROGRESS OF BIOCHIP DEVELOPMENT FOR RESEARCH AND CONSUMER MARKET
STEM CELL PROTEOMICS AND CARDIOVASCULAR DISEASE
SELECTION OF DNA APTAMERS USING ATOMIC FORCE MICROSCOPY
SINGLE-CELL TECHNOLOGY FOR REGENERATIVE CELL ENGINEERING
EMBRYOLOGY AT THE SINGLE-CELL LEVEL: GENE EXPRESSION, LINEAGE, AND CELL IDENTITY
NEW DEVELOPMENT OF LOW——COST AND HIGH——THROUGHPUT DNA SEQUENCING
MODERN RESEARCH OF TRADITIONAL CHINESE MEDICINE IN THE POST-GENOME ERA
PLrITING GENOME IN CONTEXT: TRANSCRIPTIONAL REGULATION IN THREE DIMENSIONAL ECM
MICROENVIRONMENT
CONSTRUCTION OF THE STIMULI-DEPENDENT NATURAL PROTEIN MUTANTS USING THE DE
NOVO DESIGNED LIGAND-RESPONSIBLFaz-HELICAL COILED-COIL
APPLICATIONS OF PEPTIDE NUCLEIC ACIDS FOR RNA PURIFICATION, RNA DETECTION, AND
INTRACELLULAR RNA DELIVERY
NOVEL POLYMERIC MICROFLUIDIC DEVICES FOR BIO & CHEMICAL APPLICATIONS
DNA SCAFFOLDS FOR THE ASSEMBLY OF NANOMATERIALS
HIGH THROUGHPUT DNA SEQUENCING, APPLICATIONS AND DISEASE INVESTIGATION
FAST CAPILLARY ELECTROPHORESIS SEPARATION OF DNA FRAGMENTS BASED ON A SHORT CAPILLARY
NEW DEVELOPMENTS IN MUTATION DETECTION BASED ON REAL-TIME PCR
ACCURATE AND PRECISE PROFILING OF PATHOLOGICAL PROTEOMES BY UTILIXATION OF HIGH
RESOLUTION NANO-LC/ORBITRAP/ETD
CONSTRUCYION OF ASYMMETRIC CELL——SIZED LIPID VESICLES FROM LIPID-COATED WATER-
IN-OIL MICRODROPLETS
HOMOGENEOUS DETECTION OF DNA SEQUENCES BASED ON MASS SPECTROMETRY IN
COMBINATION WITH NANOPARTICLES PROBE
STEM CELL GENE EXPRESSION PROFILING AND DIGITAL DETECTION USING A NOVEL,
ULTRASENSITIVE AND HIGHLY VERSATILE MICROFLUIDIC PLATFORM
AUTOMATION FOR MICROARRAY ANALYSIS
NEW VIRUS DISCOVERY WITH NEXT-GENERATION SEQUENCING
THE DEVELOPMENT OF A MULTIPLEX PCR METHOD AND AN AUTOMATIC DEVICE FOR
MOLECULAR DIFFERENTIAL DIAGNOSIS
HOMOGENOUS DETECTION OF MICRORNAS USING CATIONIC CONJUGATED POLYMERS AND
BRANCHED ROLLING-CIRCLE AMPLIFICATION
WHAT DO WE LEARN BY LETYING OUR GENOME OUT OF THE BOITLE
SIMPLE, CHEAP AND PRACTICAL CHIP FOR BIO-ANALYSIS OF POST GENOME AGE
CURRENT ADVANCES IN HIGH THROUGHPUT SNP GENOTYPING BASED ON HYBRIDIZATION ON
MAGNETIC NANOPARTICLES
PROGNOSDX HEALTH TISSUE —— BASED EPLIGENETIC TEST FOR PREDICTING CANCER
PROGRESSION AND RESPONSE TO THERAPY
MOLECULAR DIAGNOSIS BASED ON IMPROVED PYROSEQUENCING
NANOTECHNOLOGY AND NEW TECHNOLOGICS
A GREEN METHOD FOR FUNCTIONALIZATION OF MULTI- WALLED CARBON NANOTUBES WITH
HYDROXYL GROUPS
A AUTOMATIC DEVICE BASED ON THE IMMUNOBEAD METHOD FOR SCREENING COLORECTAL
CANCER BY EXTRACTING EXFOLIATED COLONOCYTES FROM THE HUMAN FECES
A NOVEL STRATEGY FOR ANALYZING MIRNA SEQUENCES OF SOLID SYSTEM BASED ON 2 - BASE
COLOR CODES
ANALYSIS OF COPPER ION CHANNEL PROTEIN IN BACILLUS SUBTILIS FROM BIOINFORMATICS
BIOINFORMATICAL MINING MICROSATELLITES IN GENOME OF SEA ANEMONE
DESIGN AND PREPARATION OF MONOLAYER-BARCODED NANOPARTICLES PROBE FOR DNA
DETECTION
DETECTION OF DNA HYBRIDIZATION BASED ON THE QUENCHING OF UPCONVERSION
FLUORESCENCE OF NAYF4 :YB,ER NANOSPHERES
HETEROZYGOTE DETECTION BY COLOR-CODED MINISEQUENCING
HIGH-THROUGHPUT GENOMIC DNA EXTRACTION FROM BLOOD SAMPLE USING MAGSTATION
SYSTEM
IMPROVEMENT ON SYNTHESIS OF DIFFERENT PARA-SUBSTITUTED PHENYLBORIC ACID
PREPARATION AND CItARACTERIZATION OF CS/PCL VASCULAR SCAFFOLI BY EOSPINNING
SI-NW ARRAYS DECORATED WITH GOLD NANOPARTICLES FOR SURFACE-ENHANCED RAMAN
SCATTERING
THE BIOLOGICAL FUNCTION OF NANO-DRUGS
TOTAL HCS SOLUTION: NEW CELLOMICS PLATFORM
UITRASENSITIVE DNA ELECI'ROCHEMICAL DETECTION BASED ON SILVER NANOPARTICLES
DESIGN AND APPLICATION OF THE SOFTWARE FOR COUNTING COLOR-BEADS RANDOMLY
DISTRIBUTED ON THE CHIP
MICROARRY AND MICROFLUIDICS
A GRAVITY-ACTUATED TECHNIQUE FOR DROPLET MICROFLUIDICS
A NEW MICROFLUIDIC ELECTROMICAL CELL AND APPLICATION FOR CHIP FLOW BIOSENSOR
A NOVEL KIND OF POROUS PSEUDO-CARBON PASTE ELECTRODE ELECI'ROCHEMICAL
BIOSENSOR AND ITS APPLICATION IN BIOASSAY
A SIMPLE AND RELIABLE CONFIGURATION WITH NEEDLE VALVE FOR MANIPULATING FLOW IN
MICROFLUIDIC CHIP
CELL MICROARRAY CHIP FOR THE RAPID AND BIGH-SENSITIVE DETECTION OF MALARIA-
INFECTED ERYTHROCYTES
DEVELOPMENT OF AN SEMI-AUTOMATED SYSTEM WITH A MAGNETIC NANOPARTICLES
SEPARATION DEVICE FOR SNPS DETECTION
DIRECT CHIP PCR AND ONLINE CHIP ELECTROPHORESIS WITHOUR SAMPLE PROCESSING
FABRICATION OF PLANAR MICRO-ELECTRODE ARRAYS AS A CELL-BASED BIOSENSOR
FLOW SOLID PHASE PYROSEQUENCING REACTION ON CAPILLARY MICROFLUIDIC CHIP
GLASS CHAOTIC CHIP MIXER AND USE IN CHIP FLOW CHEMILUMINESCENCE DETECTION
MICROCHIP FREE FLOW ELECTROPHORESIS (u-FFE) FOR ISO-ELECIRIC FOCUSING (IEF)
NEW NANOFLUIDIC INTERFACE FOR ONLINE PRE-CONCENTRATION OF CHARGED SPECTIES IN
CAPILLARY CHIP ELECTROPHORESIS
PREPARATION OF MESOPOROUS SILICA AND ITS EFFECT ON THE STABILITY OF CHLOROPHYLL
PREPARATION OF MONODISPERSE BIODEGRADABLE POLYLACTIC ACID MICROSPHERKS USING A
SIMPLE MICROFLUIDIC APPROACH
PROBE ARRAY DESIGN SYSTEM FOR IN-SITU SYNTHESIS OF GENE CHIPS BASED ON EXCEL AND
VISUAL BASIC FOR APPLICATIONS
SIGLE-AND MULTI-ANALYTE DETERMINATION USING A CHIP- BASED SURFACE ENHANCED
RAMAN SCATrERING SYSTEM
THE DIFFERENTIATION OF EMBRYOID BODIES INTO CARDIOMOYOCYTES USING A
MICROFLUIDIC CHIP
THE INTEGRATED DESIGN FOR MICROARRAY-IN-A-TUBE DETECTION SYSTEM
FABRICATION OF WEARABLE AG/AGCL ELECIRODES AND ITS APPLICATION IN ECG MEASUREMENT
DEVELOPMENT OF SINGLE TEMPLATE AMPLIFICATION AND PRODUCT IMMOBILIZATION ON A
SINGLE BEAD
BIOMOLECULE ANALYSIS
A BAYESIAN NETWORK-BASED APPROACH TO CONSTRUCTING GENE REGULATORY NETWORK
A GENOME ASSEMBLY PLATFORM FOR NEXT-GENERATION SEQUENCING TECHNOLOGY
A MINIATURE SPR BIOSENSOR FOR THE DETECTION OF 3-NITROTYROSINE
A NEW METHOD TO DISCOVER MICRORNAS FROM HIGN THROUGHPUT SEQUENCING DATA
BASED ON "SEED SEQUENCES" OF MICRORNAS
AN APPROACH TO SELECTION OF FUNCTIONAL SNP BASED ON ASSOCIATION BETWEEN POLY
MOREPHISM AND EXPRESSION OF GENE
ANTIHYPERTENSIVE EFFECTS OF TOTAL GINSENOSIDES AND REGULATION OF THE PERTURBED
METABOLISM OF SPONTANEOUSLY HYPERTENSIVE RATS
APPLICATION OF AN IMPROVED HIGH-SENSITIVITY PYROSEQUENCING SYSTEM FOR A
PORTABLE BIOLUMINESCENCE ANALYZER
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序言Human body consists of many organs where many cells work together to perform their functions. Although massivemolecular data are averages over ensembles of cells, the question is whether all the cells in an organ work in thesame way. In a cancer tissue, it has been pointed out that a small amount of cancer stem cells play important rolesto reconstruct a cancer tissue again even after destroying the cancer tissue by anti-cancer drugs. However, the de-tails have not been clarified because of the difficulty of analyzing very small number of cancer stem cells. It has beenalso pointed out that the heterogeneity of ES cells might be important for keeping their totipotency. For under-standing a real life system, we need new tools to analyze individual cells besides the averaged biological data. Weare at a new frontier where we understand functions of organs based on the information on individual cells usingnew tools.Seven years ago, we started IFPT to promote the interdisciplinary and international collaborations in the post ge-nome era. Beijing is the capital of China and has many beautiful places. I hope all the participants will relax and en-joy the meeting in Beijing and hopefully have chances for collaborations.
文摘There is increasing evidence that microRNAs are involved in the pathogenesis of ischemic diseases such as post-ischemic mouse hind limb, and myocardial ischemia resulted in infarction. MiR-210 expression was uregulated inresponse to hypoxia in vivo and in vitro and evidence showed that it strongly regulated human endothelial cellsinduced by hypoxia in vitro. In I/R model, the expression of miRNA-210 was increased at early time pointsfollowing I/R （1——3 days） relative to levels obtained in shams, miR-17~92 cluster is highly expressed in humanendothelial cells and that miR-92a, a component of this cluster, controls the growth of new blood vessels（angiogenesis）. Lately, Angelika Bonauer et al. identified that overexpression of miR-92a in endothelial cellsblocked angiogenesis in vitro and in vivo. In mouse models of limb ischemia and myocardial infarction, systemicadministration of an antagomir designed to inhibit miR-92a led to enhanced blood vessel growth and functionalrecovery of damaged tissue. The mouse hind limb ischemic model to study the expression level of miR-92a inskeletal muscle changes in response to ischemic injury. Ischemic injury significantly increased miR-92a expression,with a maximal effect seen within 1 to 3 days after injury and then antagomir-92a can promote neovascularization inischemic limbs.Tubular regeneration is an important element of the initial recovery from AKI induced by I/R; however, thestructural recovery of the vasculature is impaired and this may be critical in the compromise of long-term functionfollowing I/R. Although both structural and functional alterations in microvascular cells have been described withinhours of kidney I/R injury, whether the mechanisms of microvascular cells loss are related with expression ofmicroRNAs have not been delineated. Studies have suggested that miR-210 and miRNA-92a, which controlledangiogenesis and functional recovery of ischemic tissues such as hind-limp and heart in mice, were up-regulated
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